Respiratory PCR – Extended Panel

Microbiology

Multiplex Nucleic acid amplification assay (PCR) test for the detection of:

Viruses:
- SARS-CoV-2, Influenza A, Influenza B, RSV (A+B), Adenovirus, Bocavirus, Seasonal Coronaviruses (229E, HKU1, NL63, OC43), Human Metapneumovirus (A+B), Parainfluenza (1-4), Rhinovirus/Enterovirus
Bacteria:
- Bordetella pertussis, Chlamydophila pneumoniae, Legionella pneumophila, Mycoplasma pneumoniae

Diagnosis of respiratory infection caused by viruses and atypical bacteria.

Only performed after agreement with a Consultant Microbiologist.

Nasopharyngeal swab

Test sensitivity is dictated by the amount of virus on the swab and is therefore influenced by:
- (1) the timing of the test in relation to the symptoms and
- (2) the quality of the sample (operator-dependent).
- A result should be interpreted in light of the pre-test probability (severity of symptoms, their level of exposure, the disease in the contact); no test is 100% sensitive or specific.

Bronchial Alveolar Lavage (BAL) / Bronchial Washings (BAL)

Yellow topped Cobas swab (preferred)

Red/green topped viral swab

BAL/BW in Sputum pot or sterile universal

For electronic requesting using EPIC (RDUH ONLY):

Search for: ‘Respiratory Viral PCR Panel‘ or test code ‘LAB9702‘ and indicate that an extended panel is required.

Only tested after agreement with a Consultant Microbiologist or if patient meets previously agreed clinical criteria.

Processed locally in Microbiology at RDUH

Urgent – within 3 hours of sample receipt (During routine opening hours)

Non-urgent – within 24 hours

QIAGEN QIAstat-Dx