Nucleic acid amplification assay for the detection and quantification of Epstein-Barr Virus (EBV) DNA extracted from whole blood.
The assay used detects a specific target in a region of the EBNA-1 gene of EBV.
Used for detection of infection in transplant / immunocompromised patients.
EBV is a double stranded DNA virus of the Herpesvirus family; it is one of the most common viruses in humans and nearly everybody will have been infected by adulthood. Infection is usually asymptomatic in young children but delayed primary infection can cause infectious mononucleosis (“glandular fever”). After initial infection the virus will become latent in a small number of B-lymphocytes and in immunocompetent individuals will cause no further issues as periodic reactivation of the virus will be supressed by the immune system. However in immunosuppressed organ transplant recipients failure to control proliferation of the virus can lead to Post-transplant Lymphoproliferative Disorder (PTLD); this is where lymphocytes multiply out of control which can lead to conditions ranging from the hyperplasia (enlargement due to the increase of cells) of an organ or tissue to the development of a lymphoma (cancer of the lymphatic system.)
Whole blood (collected in EDTA)
EDTA (pink top) 5ml
For electronic requesting using EPIC (RDUH ONLY):
Search for: ‘EBV (Epstein-Barr virus) Quantitative PCR‘ or test code ‘LAB1373‘
Please state type of immunosuppression in the clinical details e.g. transplant patient
Please contact the laboratory to discuss any urgent requests.
Processed locally in Microbiology at RDUH
5 days
EBV viral loads will be reported in IU/ml and where appropriate a log will be given.
Lower Limit of Detection 104 IU/ml
Lower Limit of Quantification 104 IU/ml
Upper Limit of Quantitation 1,000,001 IU / mL
(Please note: the conversion factor to IU from copies is 2.9IU/copy => 1 IU/mL equals approximately 0.3 copies/mL)
ELITech ELITe BeGenius
Specimen Labelling Procedure
9018
